Figure 3.

Quantitative analysis of reverse transcription of CA mutants in target cells. HeLa-P4 cells were inoculated with the indicated HIV-1 mutants, and the second-strand transfer viral DNA products accumulated at 8 hours were quantified by PCR. In half of the samples, the reverse transcriptase inhibitor Efavirenz (EFV) was included as a control for plasmid DNA contamination carryover from the transfections used to produce the virus stocks. Error bars represent the standard deviations of triplicate infections, and the results are representative of three independent experiments.