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. 2012 Feb 27;8:14. doi: 10.1186/1744-8069-8-14

Figure 2.

Figure 2

MeCP2 is expressed in oligodendrocytes and astrocytes, but not in microglia or oligodendrocyte precursor cells, in the rat superficial dorsal horn. Confocal images of rat superficial dorsal horn sections. A, Colocalization of MeCP2 (green; Millipore antibody) and APC (red). MeCP2 can be seen within the nucleus of oligodendrocytes. B, Colocalization of MeCP2 (green; Millipore antibody), GFAP (red) and DAPI, a nuclear marker (blue). MeCP2 can be seen within the nucleus of astrocytes. C, Expression of MeCP2 (green; Millipore antibody) and IBA1 (red). MeCP2 stain cannot be seen within microglia. D, Expression of MeCP2 (green; Millipore antibody), NG2 (red) and DAPI, a nuclear marker (blue). MeCP2 stain cannot be seen within OPCs. Pictures show single focal plane. Scale bars, 1) 50 μm and 2) 20 μm.