Table 1.

Primers sequence for RT-qPCR and results of RT-qPCR analysis.

mRNA		Primers sequence	CFA		SNI
			Ispi vs Sham	Contra vs Sham	Ispi vs Sham	Contra vs Sham
MeCP2 e1	F	5'-ggagagactggaggaaaagtca-3'	137% **	158% *	74% *	ns
	R	5'-ccttcttaaacttcaggggtttc-3'
MeCP2 e2	F	5'-ctgtttgggagaagcagagg-3'	ns	134% *	85% *	ns
	R	5'-tggtagctgggatgttaggg-3'
FKBP5	F	5'-caccctgagcctggagagag-3'	ns	ns	ns	ns
	R	5'-gtgtcgccattactcgcagag-3'
SGK1	F	5'-gggctgtcttgtatgagatgc-3'	ns	ns	ns	ns
	R	5'-gtgccttgctgagttggtg-3'
SULT1a1	F	5'-ccgaggagactgtggattc-3'	ns	ns	ns	ns
	R	5'-gcatagtgggcatcaaagc-3'
DNMT1	F	5'-ggagtgtgtgaaagagaaa-3'	112% *	114% **	87% *	ns
	R	5'-tagccttcctcagacaat-3'
DNMT3a	F	5'-aattgtgtcttggtggat-3'	125% *	124% *	ns	ns
	R	5'-actgagaatttgccatct-3'
DNMT3b	F	5'-ggagacagcagacatctt-3'	ns	131% *	ns	ns
	R	5'-gtcactacagttcccattaac-3'
HDAC1	F	5'-cctcaccgaatccgaatg-3'	120% *	128% **	ns	ns
	R	5'-cgaatagaacgcaagaacttg-3'
HDAC2	F	5'-tcaagtttctacgatcaataagg-3'	ns	ns	ns	90% *
	R	5'-cttctccgacattaaatctctg-3'
HDAC5	F	5'-aagtacgttcaaggctaa-3'	117% **	114% **	77% ***	ns
	R	5'-cgactgctctcttcttaa-3'
β-Actin	F	5'-agattactgccctggctccta-3'	ns	ns	ns	ns
	R	5'-aggatagagccaccaatccac-3'
HGPRT	F	5'-aggacctctcgaagtgttggatac-3'	ns	ns	ns	ns
	R	5'-tgtagattcaacttgccgctgtc-3'

* P < 0.05; ** P < 0.01; ns: non significant. CFA N = 5 in each group and SNI N = 7 in each group