TABLE 2.

Phenotypes of altered seipin expression

Organism/cell manipulation	Phenotypes	Reference(s)
Saccharomyces cerevisiae FLD1 disruption	Shorter and more-saturated acyl chains on phospholipids	Fei et al. 2008
	Increase in PA (isolated microsomes), TAG, and SE levels	Fei et al. 2008Fei et al. 2011 PLoS Gen
	No increase in oleate incorporation into TAG and SE; presumed decrease in lipid utilization	Fei et al. 2008
	Upregulation of INO1 and OPI3	Fei et al. 2011 PLoS Gen
	Small clustered LDs wrapped in ER	Szymanski et al. 2007
	Enhanced LD fusion and supersized droplets (rescued by inositol supplementation)	Fei et al. 2008Fei et al. 2011 PLoS Gen
	Tgl3p mistargeting	Wolinski et al. 2011
	LD segregation defect	Wolinski et al. 2011
Drosophila melanogaster seipin knockout	Fat body: less neutral lipid; small droplets; decreased lipogenesis	Tian et al. 2011
	Salivary gland and midgut: larger and more-numerous LDs; PA and DGAT elevated; phenotype suppressed by DGAT knockdown
	Organism: hypersensitive to starvation; lower glucose tolerance
Mus musculusseipin knockout	Adipose tissue: decrease in WAT and BAT mass; small unilocular droplets	Cui et al. 2011
	Liver: enlarged; large increase in lipid storage
	Organism: lower body weight at birth; higher temperature; decreased adipokines; diabetic; impaired insulin signaling
HeLa seipin knockdown	Increase in TAG, LD proliferation and clustering (with exogenous oleate)	Fei et al. 2011 JLR
HaLa seipinoverexpression	Reduction in TAG with no change in FA uptake or lipolysis (with exogenous oleate)
Mouse 3T3-L1seipin knockdown	Block in terminal differentiation	Chen et al. 2009
	Increased TAG (with exogenous oleate)	Fei et al. 2011 JLR
Mouse C3H 10T1/2 seipin knockdown	Block in terminal differentiation	Payne et al. 2008
BSCL2 patient-derived cells	Fibroblasts: small numerous LDs	Szymanski et al. 2007
	Transformed lymphocytes: small numerous LDs; more-saturated FAs (presumed due to less SCD1 activity)	Boutet et al. 2009