Fig. 7.

EETs inhibited PAECs apoptosis via the JNK/c-Jun signaling pathway. A: EETs protected against the loss of mitochondrial membrane potentials evoked by SD in a JNK-dependent manner. PAECs were stained with JC-1 probe and imaged by the fluorescent microscope and quantitative analysis of the shift of mitochondrial green fluorescence to red fluorescence from six randomly selected fields obtained from each group (n = 6; *P < 0.05). B: Activation of Caspase-3 induced by starvation was repressed by adding EETs in bovine PAECs, which was relieved by a JNK inhibitor (n = 3, *P < 0.05). C: Inhibitory effects of EETs on caspase-3 activation were reversed by silencing the JNK 1/2 gene (n = 3, *P < 0.05). D: CYP2J2 overexpression repressed the activation of caspase-3 by SD through the JNK pathway (n = 3, *P < 0.05).