Fig. 1.

Simvastatin and DPDMP affect membrane composition. HEKOPRM1 cells were treated with PBS (Control), DMSO (DMSO), 0.5 μM simvastatin (Simvastatin), 0.5 μM simvastatin with 40 ng/ml cholesterol (Simva+Chol), 40 ng/ml cholesterol (Cholesterol), or 20 μM DPDMP (DPDMP) for 12 h. The cholesterol levels were determined in whole-cell lysis and on cell membrane in (A). The amounts of membrane GM1 were determined by FACS with CTX-B-FITC in (B). Data were analyzed by one-way ANOVA with Dunnett's posthoc test. Error bars and asterisks represent SD (n ≥ 4) and significant difference from Control, respectively.