Skip to main content
. 2012 Jun;53(6):1153–1162. doi: 10.1194/jlr.M024455

Fig. 4.

Fig. 4.

Simvastatin and DPDMP affect Gαi2 coupling differentially. HEKOPRM1 cells were transfected with CFPOPRM1 and YFPGαi2. One day after the transfection, the cells were treated PBS (Control), DMSO (DMSO), 0.01 μM simvastatin (0.01 µM Simva), 0.03 μM simvastatin (0.03 µM Simva), 0.12 μM simvastatin (0.12 µM Simva), 0.5 μM simvastatin (0.5 µM Simva), 2 μM simvastatin (2 µM Simva), 0.5 μM simvastatin with 40 ng/ml cholesterol (Simva+Chol), 40 ng/ml cholesterol (Cholesterol), or 20 μM DPDMP (DPDMP) for 12 h. The normalized net FRET was determined on the cell membrane as described in Materials and Methods. Data were analyzed by one-way ANOVA with Dunnett's posthoc test. Error bars and asterisks represent SD (n ≥ 12) and significant difference from Control, respectively.