Fig. 2.

Short-term UVA irradiation has minimal effect on the levels of unoxidized PCs. Human dermal fibroblasts were irradiated for 10 (40 J/cm²) or 20 (80 J/cm²) min. The incubation was stopped by immediate washing and the addition of acidic methanol. Lipid extract from cells grown on two 35 mm wells (1/1,200^th) was injected for analysis of unoxidized species. The elution/detection was performed within 15 min. The relative amounts of unoxidized PCs were determined by the Analyst 1.5 software as areas of peaks identified using commercially available standards. There was a trend for decreased levels of PLs at the 80 J/cm² fluence; however, the differences were not statistically significant. Error bars indicate standard deviations of analytical triplicates (corresponding to six biological replicates).