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. Author manuscript; available in PMC: 2012 May 15.
Published in final edited form as: Biochem J. 2011 May 1;435(3):711–722. doi: 10.1042/BJ20101726

Figure 3. Mechanistic basis for Ca2+/CaM-dependent Cx50 gap junction uncoupling.

Figure 3

(A) The product of the number of open channels (N) and open probability (P o) from two poorly coupled Cx50-N2a cell pairs is plotted as a function of time and 1 μM ionomycin +1.8 mM [Ca2+]o superfusion. N · Po declined from an average control value of 1.21 open channels to 0 within 2 min of ionomycin/CaCl2 saline superfusion. (B) Cx50 gap junction channel current recordings from one experiment are shown for the time points indicated by the circles in (A). The number and duration of the open channels declined progressively to 0 without any apparent reduction in the single-channel conductance (314 pS), indicative of closure of a Ca2+–CaM-dependent gate without a block of the ion-permeation pathway.