Figure 6. CD studies of the interaction between CaM and Cx50p^{141 – 166}.

(A) Far-UV CD spectra of CaM in the presence of 5 mM EGTA (○, apo-CaM) or CaCl₂ (□, holo-CaM), and a CaM–Cx50p^141–166 (1:1) mixture with 5 mM EGTA (●, apo-CaM–Cx50p^141–166) or CaCl₂ (■, holo-CaM–Cx50p^141–166). (B) Far-UV spectra of the synthetic peptide Cx50p^141–166 with addition of TFE (0–80 %). The inset shows calculated α-helical content as a function of TFE concentration for the peptides Cx50p^141–166 (■), Cx44p^129–150 (▲) and Cx43p^136–158 (●). (C) Far-UV spectra of the synthetic peptide Cx50p^141–166 with (●) or without (○) addition of CaM.