Skip to main content
. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Jan 14;1822(4):589–599. doi: 10.1016/j.bbadis.2012.01.008

Fig. 5.

Fig. 5

RNA-Seq identifies a TGF-β1 regulated gene set with overlapping expression profile in human DN. (A) 2027 genes were identified as significantly differentially expressed in renal epithelial cells in response to TGF-β1 treatment. Heatmap of gene expression patterns of 1356/2027 genes available from microarray data of renal biopsies from human diabetic nephropathy (DN; n=11) versus living donor (LD; n=3) and minimal change disease (MCD; n=4) controls. (B) A subset of 179 genes were significantly differentially expressed in both RNA-Seq and human DN microarray datasets. Heatmap of log-fold changes of 179 genes in HK-2 cells stimulated with TGF-β1 (5ng/ml; 48hr), and gene expression patterns of corresponding 179 genes from microarray data of renal biopsies from human diabetic nephropathy (DN; n=11) versus living donor (LD; n=3) and minimal change disease (MCD; n=4) controls. Microarray data are log2 median-centred intensities. Green – downregulated gene expression; Red – upregulated gene expression.