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. 2011 Dec 6;22(1):219–236. doi: 10.1038/cr.2011.195

Figure 4.

Figure 4

Rescue of innate cardiac deficiency by AA treatment. (A) AA induced the development of contracting EBs in other 5 iPSC lines without spontaneous cardiogenic potential. Data were collected at day 10. (B) Differentiation profile of cardiomyocytes in three representative iPSC lines. (C) Quantitative RT-PCR analysis of the expression level of cardiac genes. (D) Immunostaining showing the only emergence of α-actinin+ and cTnT+ cardiomyocytes in AA-treated EBs at day 10. Scale bars = 100 μm. Nuclei were counterstained with Hoechst33258 (blue). Data are expressed as means ± SEM. *P < 0.05, **P < 0.01 vs control.