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Tumorigenic spheres are derived from the peripheral blood of gastric cancer patients. (A) CD44- and CD54-positive cells were sorted, stained with anti-pan-cytokeratin (PAN-CK) antibody and DAPI, and visualized by fluorescence microscopy. (B) Spheres were generated from fewer than 103 CD44-positive cells. Primary sphere: original spheres formed from blood cells. Second sphere: second passage of spheres. (C) An example of a mouse bearing a tumor (arrow) produced by blood-borne spheres.
