Skip to main content
. 2011 Aug 9;22(1):237–247. doi: 10.1038/cr.2011.127

Figure 1.

Figure 1

Identification of SKL2001 as a small-molecule activator of Wnt/β-catenin signaling. (A) Screening of compounds that inhibit Wnt/β-catenin signaling. (B) Chemical structure of SKL2001. (C) Dose-dependent response for CRT activation with increasing concentrations of SKL2001. HEK293 reporter and control cells were incubated with the indicated concentrations of SKL2001 for 15 h and luciferase activity was determined. The results are shown as the average of three experiments; the bars indicate standard deviations. (D) Cytosolic and nuclear proteins were prepared from HEK293 reporter cells treated with either vehicle (DMSO), Wnt3a CM, LiCl (20 mM) or SKL2001 (10 and 30 μM) and were then subjected to western blotting with anti-β-catenin antibody. The blots were reprobed with anti-actin antibody as a loading control. (E) Semi-quantitative RT-PCR for β-catenin, and GAPDH was performed with total RNA prepared from HEK293 reporter cells treated with the vehicle (DMSO) or indicated concentrations of SKL2001 for 15 h.