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. 2011 Sep 20;22(1):107–126. doi: 10.1038/cr.2011.158

Figure 6.

Figure 6

Exogenous MIR168a inhibits mouse liver LDLRAP1 expression and elevates plasma LDL-cholesterol level at 3 days after food intake. (A) The weight changes of mice fed with chow diet or fresh rice (n = 8). (B) The food intake of chow diet or fresh rice (n = 8). (C, D) The levels of MIR168a in mouse sera (C) and livers (D) after chow diet or fresh rice feeding (n = 8). (E) The LDLRAP1 protein levels in mouse livers after chow diet or fresh rice feeding. (F) The quantification of LDLRAP1 protein expression in E (n = 8). (G) The levels of LDL-cholesterol in mouse plasma after chow diet or fresh rice feeding (n = 8). (H, I) The levels of MIR168a (H) and LDLRAP1 protein (I) in mouse livers after 3 days of feeding with chow diet, fresh rice, or fresh rice accompanying an injection of anti-MIR168a ASO or anti-ncRNA (n = 8). (J) The quantification of LDLRAP1 protein expression in I (n = 8). (K) The levels of LDL-cholesterol in mouse plasma after 3 days of feeding with chow diet, fresh rice, or fresh rice accompanying an injection of anti-MIR168a ASO or anti-ncRNA (n = 8). (L) The association of MIR168a with AGO2 in mouse livers after 3 days of feeding with chow diet, fresh rice, or fresh rice plus injection of anti-MIR168a ASO or anti-ncRNA. The levels of MIR168a and miR-16 (control) in anti-AGO2 immunoprecipitated products were detected by qRT-PCR. (M) The association of LDLRAP1 mRNA with AGO2 in mouse livers after 3-day feeding. The LDLRAP1 mRNA in anti-AGO2 immunoprecipitated products was detected by semi-quantitative RT-PCR. (N, O) The levels of MIR168a (N) and LDLRAP1 protein (O) in mouse livers after 3 days of feeding with chow diet and mature MIR168a or ncRNA (n = 8). (P) The quantification of LDLRAP1 protein expression in O (n = 8). (Q) The levels of LDL-cholesterol in mouse plasma after 3 days of feeding with chow diet and mature MIR168a or ncRNA (n = 8). *P < 0.05; **P < 0.01.