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. 2012 Apr 9;5:11. doi: 10.1186/1756-6606-5-11

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Copyright ©2012 Belrose et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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TRPM2 mRNA levels do not change in hippocampal primary cultures with time in vitro. (A) TRPM2 mRNA was measured at 14, 21, and 29 DIV (n = 8 per group) by quantitative real-time PCR (qPCR). No significant change in cycle threshold values is observed (p = 0.89) (B) QPCR analysis of neuron specific enolase (ENO2) mRNA values used to control for neuronal content over time. No significant difference was observed (p = 0.9). (C) Normalized TRPM2 values for each sample (ENO2-TRPM2) were quantified. No difference in the normalized levels of TRPM2 mRNA is seen over time in culture (p = 0.51).