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. 2012 Feb 15;12:22. doi: 10.1186/1471-2229-12-22

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Copyright ©2012 Feng et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gene expression, sequence analysis and subcellular localization of MdCIbHLH1.(A) Semi-quantitative RT-PCR analysis of MdCIbHLH1 at 25°C and 4°C. The apple tissue cultures were treated for 4 h. (B) Genomic structure of MdCIbHLH1, AtICE1 and AtICE2. (C) Phylogenetic tree containing MdCIbHLH1 and the bHLH proteins of Arabidopsis. The solid circle represents MdCIbHLH1, while empty cycles represent the Arabidopsis ICE1 and ICE2 bHLH transcription factors. (D) The bHLH domain is highly conserved in MdCIbHLH1 and the other bHLH TFs (AtICE1: AT3G26744; AtICE2: AT1G12860; CbICE1: AAS79350; GmICE1: ACJ39211; OsICE1: NP_001045272; PtICE1: ABF48720; PsICE2: ADK91821; GmICE4: ACJ39214; PsICE1: ABF48720; ThICE1: BAJ34291; GmICE2: ACJ39212; GmICE3: ACJ39213) proteins. The bit score indicates the information content for each position in the sequence. (E) Three onion epidermal cells producing GFP alone. (F) An onion epidermal cell producing the MdCIbHLH1-GFP fusion protein.