Figure 7.

Cold-induced degradation and post-translational modification of MdCIbHLH1. (A) Detection of MdCIbHLH1-GFP protein by Western blot analysis. TGC calluses were maintained at 15°C for the cold treatment. MdCIbHLH1-GFP protein levels were analyzed using anti-GFP. Nonspecific Coomassie blue staining of a duplicate gel shows that similar amounts of proteins were loaded. (B) Detection of immunoprecipitated MdCIbHLH1-GFP protein by SDS/PAGE. (C) In vivo detection of ubiquitinated and sumoylated MdCIbHLH1-GFP in TGC calluses.