Table 3.
Summary of proteomics data from the reduction and differential Cys-labelling of 2B4 cells with Thioredoxin. The modified Cys residues are indicated by residue number (@ followed by residue number in peptide) and whether the modification detected was MPB itself or a hydrolysis derivative (indicated by +H2O). Protein probability scores from iProphet meta-searches are shown and where applicable weighted spectral index counts (WSC) are shown for the reduced and control samples, respectively. The percentage sequence coverage indicates the percentage of the protein sequence observed. Cys denotes the modified Cysteine number in the protein sequence inclusive of the signal peptides.
| IPI accession | gene | protein description | protein identification probability | % sequence coverage | WSC control | WSC TRX reduced | maleimide-modified peptide | modification | Cys |
|---|---|---|---|---|---|---|---|---|---|
| IPI00123329 | Adam15 | ADAM15 | 1 | 13.6 | TRX only | ||||
| IPI00381630 | Adam17 | ADAM17 | 1 | 12.8 | TRX only | ||||
| IPI00279010 | Bcam | CD239, BCAM | 1 | 17.6 | TRX only | ||||
| IPI00113869 | Bsg | CD147, Basigin | 1 | 46.9 | 6 | 30 | |||
| IPI00108001 | Cd2 | CD2 | 1 | 20.3 | TRX only | CEAINPVSK | MPB@1 | 180 | |
| IPI00119703 | Cd244 | CD244, 2B4 | 1 | 22.1 | TRX only | ||||
| IPI00114509 | Cd3d | CD3 delta | 1 | 26 | TRX only | ||||
| IPI00223769 | Cd44 | CD44 | 1 | 11.5 | 2 | 10 | |||
| IPI00124830 | Cd47 | CD47 | 1 | 14.8 | 1 | 11 | TAFNTDQGSACSYEEEK | MPB+H2O@11 | 142 |
| IPI00380293 | Cd96 | CD96 | 1 | 3.2 | TRX only | ||||
| IPI00121627 | Clptm1 | cleft lip and palate transmembrane protein 1 homologue | 1 | 19.4 | 1 | 21 | VAGIFPCPTFK | MPB+H2O@7 | 454 |
| IPI00420148 | Env | GP160 | 1 | 43.9 | 12.98 | 190.91 | WGCETTGQAYWKPSSSWDLISLK | MPB+H2O@3 | 131 |
| CNPLVLEFTDAGK | MPB+H2O@1 | 181 | |||||||
| THQALCNTTQK | MPB@6 | 368 | |||||||
| CNPLVLEFTDAGKK | MPB@1 | 181 | |||||||
| LTLSEVTGQGLCVGAVPK | MPB+H2O@12 | 356 | |||||||
| TFDFYVCPGHTVPTGCGGPR | MPB@7 | 100 | |||||||
| EGGLCAALKEECCFYADHTGVVR | MPB+H2O@12 | 533 | |||||||
| IPI00126300 | H2-D1 | H-2 class I histocompatibility antigen, D-K alpha chain | 1 | 26.8 | TRX only | ||||
| IPI00114492 | H2-K1 | H-2 class I histocompatibility antigen, K-B alpha chain | 1 | 29.4 | TRX only | ||||
| IPI00129526 | Hsp90b1 | endoplasmin | 1 | 58.1 | 28.94 | 164.84 | |||
| IPI00323357 | Hspa8 | heat shock cognate 71 kDa protein | 1 | 50.5 | 2.99 | 41.74 | |||
| IPI00880839 | Hspa9 | stress-70 protein | 1 | 75.7 | 5 | 288 | MEEFKDQLPADECNK | MPB@13 | 608 |
| DQLPADECNK | MPB@8 | 608 | |||||||
| AKCELSSSVQTDINLPYLTMDASGPK | MPB+H2O@3 | 317 | |||||||
| GAVVGIDLGTTNSCVAVMEGK | MPB+H2O@14 | 66 | |||||||
| CELSSSVQTDINLPYLTMDASGPK | MPB@1 | 317 | |||||||
| IPI00129679 | Ifngr1 | CD119, interferon gamma receptor 1 | 1 | 5 | TRX only | YCISVDGISSFWQVR | MPB+H2O@2 | 223 | |
| IPI00321348 | Igsf8 | CD316, immunoglobulin superfamily member 8 | 1 | 6.2 | TRX only | ||||
| IPI00119612 | Il2rg | CD132, cytokine receptor common subunit gamma | 1 | 30.9 | TRX only | ||||
| IPI00120155 | Il6st | CD130, interleukin-6 receptor subunit beta | 1 | 13.2 | TRX only | ||||
| IPI00132286 | Itgal | integrin alpha-L | 1 | 49.4 | 27.88 | 90.63 | GSLLACDPGLSR | MPB+H2O@6 | 108 |
| IPI00120245 | Itgav | integrin alpha-V | 1 | 9.5 | 2 | 7.99 | |||
| IPI00132474 | Itgb1 | integrin beta-1 | 1 | 37.1 | TRX only | LGGIVLPNDGQCHLENNVYTMSHYYDYPSIAHLVQK | MPB+H2O@12 | 299 | |
| IPI00877242 | Itgb3 | integrin beta-3 | 1 | 7 | TRX only | ||||
| IPI00310109 | Lamp2 | lysosome-associated membrane glycoprotein 2 | 1 | 18.8 | TRX only | NLSFWDAPLGSSYMCNK | MPB+H2O@15 | 336 | |
| IPI00785217 | Ldlr | low-density lipoprotein receptor | 1 | 41.3 | 7.93 | 83.26 | TTEDELHICR | MPB+H2O@9 | 843 |
| IPI00119809 | Lgals3bp | galectin-3-binding protein | 1 | 34.3 | 3 | 32 | |||
| IPI00761657 | Lgals8 | galectin-8 | 1 | 16.1 | TRX only | SSCIVCNTLTQEK | MPB+H2O@3 | 77 | |
| IPI00114396 | Lgals9 | galectin-9 | 1 | 54 | 7 | 77 | GMPFELCFLVQR | MPB+H2O@7 | 101 |
| FEEGGYVVCNTK | MPB+H2O@9 | 73 | |||||||
| IPI00606283 | LOC665 506 | TCR chain | 1 | 47.3 | 1 | 27.77 | |||
| IPI00121600 | Lrp8 | low-density lipoprotein receptor-related protein 8 | 1 | 24.1 | TRX only | ||||
| IPI00129253 | Ly75 | CD205, CLEC13B | 1 | 10.2 | TRX only | ||||
| IPI00129646 | Ly9 | CD229, LY-9 | 1 | 36.3 | 8 | 49 | DAEIEHIIWNCPPK | MPB+H2O@11 | 82 |
| IPI00108844 | M6pr | CD222, cation-independent mannose-6-phosphate receptor | 1 | 36 | TRX only | AVVMISCNR | MPB+H2O@7 | 146 | |
| IPI00125890 | Pdcd1 | CD279, PD-1 | 1 | 24 | 2 | 17 | QAAFCNGLSQPVQDAR | MPB+H2O@5 | 84 |
| IPI00230108 | Pdia3 | PDI-A3 | 1 | 33.7 | 6 | 23 | |||
| IPI00271951 | Pdia4 | PDI-A4 | 1 | 7.3 | TRX only | ||||
| IPI00177179 | Pvr | CD155, poliovirus receptor | 1 | 12.7 | TRX only | ||||
| IPI00116921 | Scarb1 | CD36L1, SCARB-1 | 1 | 14.4 | TRX only | EHSLFLDIHPVTGIPMNCSVK | MPB+H2O@18 | 385 | |
| IPI00127447 | Scarb2 | CD36L2, SCARB-2 | 1 | 45.6 | TRX only | TSLDWWTTDTCNMINGTDGDSFHPLISK | MPB@11 | 245 | |
| IPI00318993 | Sell | CD62L, L-selectin | 1 | 6.2 | TRX only | ||||
| IPI00464135 | Sema4b | semaphorin-4B | 1 | 10.8 | TRX only | LWVHNGAPVNASASCR | MPB+H2O@15 | 620 | |
| IPI00890869 | Sema4c | semaphorin-4C | 1 | 6.5 | TRX only | ||||
| IPI00454115 | Sema4d | semaphorin-4D | 1 | 20 | TRX only | ||||
| IPI00131832 | Slamf1 | CD150, SLAM | 1 | 7.1 | TRX only | ||||
| IPI00315758 | Slc11a2 | divalent cation transporter 1 | 1 | 7.7 | TRX only | LGVVTGLHLAEVCHR | MPB+H2O@13 | 137 | |
| IPI00120769 | Slc29a1 | equilibrative nucleoside transporter 1 | 1 | 7.4 | TRX only | IVFIPLLMLCNVK | MPB+H2O@10 | 378 | |
| IPI00120166 | Slc30a1 | zinc transporter 1 | 1 | 4 | TRX only | ||||
| IPI00459577 | Slc38a1 | sodium-coupled neutral amino acid transporter 1 | 1 | 10.8 | TRX only | TVYALPTIAFAFVCHPSVLPIYSELK | MPB+H2O@14 | 286 | |
| IPI00273801 | Slc39a10 | zinc transporter ZIP10 | 1 | 11.5 | TRX only | ||||
| IPI00114641 | Slc3a2 | CD98, 4F2 heavy chain | 1 | 82.3 | 38 | 212 | |||
| IPI00121634 | Slc7a1 | high-affinity cationic amino acid transporter 1 | 1 | 8.5 | TRX only | TPDSNLDQCK | MPB+H2O@9 | 621 | |
| IPI00331577 | Slc7a5 | 4F2 light chain | 1 | 23.2 | 0.5 | 19 | |||
| IPI00221632 | Slc7a6 | Y + L amino acid transporter 2 | 0.9997 | 9.1 | TRX only | ||||
| IPI00420955 | Sort1 | sortilin | 1 | 6.7 | 1 | 2 | |||
| IPI00124700 | Tfrc | CD71, transferrin receptor protein | 1 | 69.7 | 30 | 218 | VEQKEECVK | MPB+H2O@7 | 98 |
| WNIDSSCK | MPB+H2O@7 | 365 | |||||||
| IPI00114457 | Tgfb1 | transforming growth factor beta-1 | 1 | 35.6 | TRX only | ||||
| IPI00109727 | Thy1 | CD90, Thy-1 | 1 | 17.9 | TRX only | ||||
| IPI00133834 | Tnfrsf18 | CD357 | 1 | 13.5 | TRX only | ||||
| IPI00122738 | Trbv5 | T cell receptor beta chain V region | 1 | 33.9 | TRX only | FIPECPDSSK | MPB+H2O@5 | 86 |