Table 1. Inhibition of attachment of N. fowleri to ECM components.
N. fowleri (2×105) was preincubated (10 min) with anti-KLH or monoclonal anti-β1 integrin antibody prior to addition to ECM components for 50 min. Unattached amoebae were then collected, fixed with glutaraldehyde and counted using a haemocytometer. The percentage of bound amoebae was calculated using the following formula: percentage bound = [(2×105 amoebae)−(number of amoebae recovered)/(2×105 amoebae)]×100 %. One hundred per cent bound would indicate no amoebae recovered.
| ECM component | No antibody | Anti-KLH | Anti-β1 integrin | |||
| Amoebae recovered | Percentage bound | Amoebae recovered | Percentage bound | Amoebae recovered | Percentage bound | |
| Laminin-1 | 5.0×104 | 75 % | 7.0×104 | 65 % | 1.2×105 | 40 % |
| Collagen I | 5.0×104 | 75 % | 7.0×104 | 65 % | 1.4×105 | 30 % |
| Fibronectin | 4.0×104 | 80 % | 6.0×104 | 70 % | 1.0×105 | 50 % |