Figure 2.

The assay is non-toxic to parasite cultures. (A) Growth of indicated parasite lines over five days in the presence of 10 μM c-SNARF-1, quantified with SYBR Green I and normalized to 100% growth for controls grown without c-SNARF-1. Bars represent mean ± S.E.M of three to four trials each. (B) Normalized parasite growth in HEPES- and NaHCO₃-buffered media with indicated initial pH values (n = 3). Cultures were initiated at 1% synchronous ring-stage parasitaemia and assessed with SYBR Green I after 72 h.