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. 2012 Jan 31;56(1):e2. doi: 10.4081/ejh.2012.e2

Figure 2.

Figure 2

Morphometric analysis of neonatal rat heart performed on hematoxylin-eosin stained slides; a) ambient air; b) 60% hyperoxia; c) 95% hyperoxia; d) ambient air 6 weeks; e) 60% hyperoxia and ambient air 4 weeks. A) Fiber diameter measurements expressed as mean values (±SD) assessed by direct visual measurement of ten muscle fibers for each of three slides per each of 5 samples at 40× magnification; *ambient air 6 weeks vs ambient air P<0.05; *60% hyperoxia and ambient air 4 weeks vs ambient air P<0.05. B) Blood vessels, connective and muscle compartment measurements expressed as % area mean (±SD) assessed by direct visual counting of ten fields for each of three slides per each of 5 samples; *60% hyperoxia connective compartment % area vs ambient air connective compartment % area P<0.05; *95% hyperoxia connective compartment vs ambient air connective compartment % area P<0.05; ** 95% hyperoxia blood vessels % area vs ambient air blood vessels % area P<0.05. C) Western blotting analysis of collagen III and α-actin expression; each membrane has been probed with anti β-tubulin antibody to verify loading evenness. The most representative out of three separate experiments is shown. Data are the densitometric measurements of protein bands expressed as Integrated Optical Intensity (IOI) mean (±SD) of three separate experiments; **60% hyperoxia collagen III vs ambient air collagen III P<0.05; **95% hyperoxia collagen III vs ambient air collagen III P<0.05; **60% hyperoxia collagen III vs 95% hyperoxia collagen III; ***95% hyperoxia α-actin vs ambient air α-actin P<0.05.