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Confocal optical sections after immunolabeling for Tanks (red fluorescence) and lysosomes (green fluorescence): a) control; b) HeLa cells after treatment with 1 µg/mL actinomycin D for 20 h. DNA was counterstained with Hoechst 33258 (blue fluorescence). Lines in a, b represent the region where fusion of green/red fluorescence was measured. Upper (a',b') and lower (a'',b'') panels refer to green and red fluorescence, respectively. Scale bar: 20 µm.
