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. 2011 Sep;2(9):889–899. doi: 10.1177/1947601911432495

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Figure 1. — Silencing of p21 prevents FDH-induced G1 arrest and accelerates apoptosis in A549 cells. (A) Levels of p21 mRNA in A549 cells after transfection with p21-specific siRNA and FDH induction. (B) Levels of FDH and p21 proteins (Western blot) at different times of FDH induction in untreated (control), treated with scrambled siRNA (Scr), and treated with p21-specific siRNA (siRNA). Actin is shown as loading control. (C) Distribution of cells between cell cycle phases in p21-proficient cells without FDH (top), p21-proficient cells after FDH induction (middle), and p21-silenced cells after FDH induction (bottom). Assay was carried out at 48 hours after FDH induction. (D) Apoptosis evaluated by annexin V/PI staining in p21-proficient (scrambled) and silenced (siRNA) cells in the presence (+) and absence (–) of FDH. (E) Calculation of apoptotic cells (% of the total cells) from D. Error bars represent ±SD (n = 3).