HOXC8 and CDH11 expression are well correlated in breast cancer cells. (A) Hs578T and MDA-MB-231 cells were transduced with empty lentiviral vector or vectors encoding scramble sequence, HOXC8 shRNAs, or HOXC8 transgene. Total RNA was isolated from these cells and subjected to qRT-PCR to determine the level of CDH11 mRNA. GAPDH mRNA was used as an internal control for standardization. Data are mean ± SE. n = 4. *P < 0.005 versus empty vector. **P < 0.01 versus empty vector. (B) Hs578T and MDA-MB-231 cells transduced with lentiviral vector encoding scramble sequence or HOXC8 shRNAs were lysed and cell lysates were subjected to immunoblotting to detect HOXC8, CDH11, and β-actin. (C) Overnight cultured cells were lysed and cell lysates were subjected to immunoblotting to detect HOXC8, CDH11, E-cadherin, and β-actin. (D) The continuous sections were prepared from paraffin-embedded normal breast and breast cancer tissues. Sections were subjected to immunohistochemistry with HOXC8 and CDH11 antibodies as well as H&E staining. Scale bar, 100 μm.