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. 2011 Sep;2(9):880–888. doi: 10.1177/1947601911433129

Figure 2.

Figure 2.

HOXC8 regulation of cell migration is CDH11-dependent. (A) Hs578T or MDA-MB-231 cells were transduced with lentiviral vectors containing scramble sequence, CDH11, or HOXC8 shRNA individually or in combination for 4 days. The population of transduced cells was analyzed for cell migration by Transwell assay. Data are mean ± SE. n = 4. *P < 0.001 versus scramble. **P < 0.005 versus scramble. (B) CDH11 transgene was expressed in Hs578T or MDA-MB-231 cells that lentivirally expressed scramble sequence or HOXC8 shRNAs. Cells were analyzed for cell migration by Transwell assay. Data are mean ± SE. n = 4. *P < 0.001 versus scramble. **P < 0.005 versus scramble. (C) Hs578T cells lentivirally expressing scramble sequence, CDH11, or HOXC8 shRNA were stained with rhodamine-conjugated phalloidin. F-actin was visualized under a fluorescence microscope. Cells expressing GFP were lentivirally transduced. (D) CDH11 transgene was introduced into HOXC8-knockdown Hs578T cells. The cells were stained with rhodamine-conjugated phalloidin, and polymerized actin was visualized under a fluorescence microscope. Cells expressing GFP were lentivirally transduced.