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. 2011 Sep;2(9):880–888. doi: 10.1177/1947601911433129

Figure 5.

Figure 5.

Trio is required for endogenous Rac activity in breast cancer cells. (A) Hs578T and MDA-MB-231 cells were lentivirally transduced with vector encoding scramble sequence, Trio, or Tiam1 shRNAs for 4 days and then analyzed for Rac activities. Cell lysates were also collected and subjected to immunoblotting to detect Trio, Tiam1, and β-actin. (B) Hs578T and MDA-MB-231 cells lentivirally expressing scramble sequence or Trio shRNA were subjected immunostaining with rhodamine-conjugated phalloidin. Polymerized actin was visualized under a fluorescence microscope. Cells expressing GFP are lentivirally transduced. (C) Hs578T and MDA-MB-231 cells lentivirally expressing scramble sequence or Trio shRNA were assayed for cell migration by Transwell assay. Data are mean ± SE. n = 4. **P < 0.005 versus scramble.