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. 2011 Sep;2(9):880–888. doi: 10.1177/1947601911433129

Figure 6.

Figure 6.

CDH11 interacts with Trio in the plasma membrane regions. (A) Hs578T and MDA-MB-231 cells were lysed and cell lysates were immunoprecipitated with either Trio polyclonal antibody or CDH11 mAb. Immunoblotting was performed to detect CDH11 in Trio immunoprecipitate (left panel) and Trio in CDH11 immunoprecipitate (right panel). IgG was used as negative control. (B) Plasma membrane and cytosolic fractions of Hs578T and MDA-MB-231 cells were immunoprecipitated with either Trio polyclonal antibody or CDH11 mAb. Immunoblotting was performed to detect Trio in CDH11 immunoprecipitate (left panel) and CDH11 in Trio immunoprecipitate (right panel). IgG was used as negative control. (C) Plasma membrane and cytosolic fractions of Hs578T and MDA-MB-231 cells were subjected to immunoblotting to detect CDH11, αv, integrin, and β-actin with the respective antibodies.