Figure 7.

CDH11 facilitates the plasma membrane localization of Trio. (A) Hs578T cells were treated with control or CDH11 siRNA for 3 days and then fixed and stained with anti-Trio polyclonal antibody. The subcellular localization of Trio was visualized under a fluorescence microscope. (B) Plasma membrane fractions were prepared from Hs578T and MDA-MB-231 cells lentivirally expressing scramble sequence or CDH11 shRNAs and then subjected to immunoblotting to detect Trio and αv integrin subunit. Whole cell lysates were also collected and subjected to immunoblotting to detect Trio, CDH11, and β-actin.