Table 2. Role of quorum sensing in expression from the A1S_0112 promoter.
Strain | Growth condition* | β-Galactosidase activity (Miller units) |
M2 wild-type/A1S_0112-lacZ | LB | 8.7±0.7 |
M2 wild-type/A1S_0112-lacZ | LB+AHL | 15.6±0.9 |
M2 abaI : : Km/A1S_0112-lacZ | LB | 2.1±0.1 |
M2 abaI : : Km/A1S_0112-lacZ | LB+AHL | 15.0±0.5 |
Cells were harvested at OD600 0.6. Supplementation of LB was with 10 µM 3-OH C12-HSL (AHL).