Fig. 1.

Overview of E. coli K-12 MG1655 A-stat experiment in which samples for HT DNA sequencing were taken. Grey dots denote sampling points for HT DNA sequencing: stock culture and two samples at D = 0.11 and 0.48 h⁻¹. The numbers of generations between the sampling points were as follows: stock culture and D = 0.11 h⁻¹, 18 generations (including four generations of continuous culture); D = 0.11 and 0.48 h⁻¹, 16 generations. The glucose concentration remained below the detection limit (120 mg l⁻¹) until the maximal specific growth rate was achieved. SC, stock culture; PC, pre-culture; X, dry cell weight (DCW) per litre; D, dilution rate; Y_OAc⁻, acetate production per gram dry cell weight; CO₂, carbon dioxide volumetric percentage in the gas outflow. This A-stat experiment is the same as that reported in Fig. 1 of Valgepea et al. (2010).