Fig. 2.

Population heterogeneity in the E. coli K-12 MG1655 A-stat experiment. All the subpopulations shown here were verified with Sanger sequencing. In the stock culture, the mutation in glyA was not detected with HT DNA sequencing; therefore, the frequency for this sample was estimated from genome coverage at the position where a glyA mutation was detected in other samples. See Table 2 for details of mutations.