Fig. 2.

DNA alignment of the four V. anguillarum qrr genes and their promoters. The qrr1, qrr2, qrr3 and qrr4 genes and their 5′- and 3′-flanking sequences were aligned using clustal w (Thompson et al., 1994). Regions of the gene sequences with 100 % identity are highlighted in grey. In each promoter region, consensus σ⁵⁴ promoter sequences were found and designated −24 and −12. The consensus σ⁵⁴ promoter sequence (YTGGCACG-N4-TTGCWNN, Barrios et al., 1999) is given below the aligned sites. Putative transcriptional start sites are designated +1. Putative LuxO binding sites were found upstream of the promoters and are indicated with a box. The consensus sequence for the LuxO binding site (TTGCA-N₃-TGCAA, Lenz et al., 2004) is shown below the aligned sites. Possible stem–loop transcriptional terminators are indicated with solid lines.