Fig. 2.

Disrupting the fumarate reductase complex reduces the rate of ROS formation in B. fragilis. Cells grown to exponential phase were centrifuged, resuspended in AMM lacking cysteine, and shaken for 1 h at 37 °C under room air. Cells were again centrifuged, washed and resuspended in PBS+thymine to OD₆₀₀ 0.1. Freshly prepared glucose was added to 0.05 % just before the start of assay. Suspensions were shaken at 37 °C under room air throughout the course of the assay. Samples were taken over time and cells were centrifuged. Supernatants were assayed for H₂O₂ using the AR protocol. BM105 (ΔahpCΔkatΔtpx, ▪), BM112 (ΔahpCΔkatΔtpxΔfrdC, ▴, dashed line). Shown are the means of three assays±sem.