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Albumin depletion abrogates serum enhancement of PAO1 gene expression. DEAE Affi-Gel Blue gel affinity columns (Bio-Rad) were used to deplete ABS (B-AD) and AHS (H-AD) of albumin. PAO1/pPMP220 : : PpvdS was grown in (a) TSB-DC, TSB-DC/ABS or TSB-DC/B-AD; or (b) TSB-DC, TSB-DC/AHS or TSB-DC/H-AD. Samples were obtained at 2 and 4 h and the level of β-galactosidase activity was determined. Values represent the means±sem of three independent experiments. ***, P<0.001.
