Table 1. Strains and plasmids used in this study.
Strain or plasmid | Description* | Source and reference |
Strains | ||
PAO1 | Prototrophic PAO1 | S. E. H. West, University of Wisconsin (West et al., 1994) |
C6 | PAO1 parent strain; Δanr, Fur mutation is Ala-10-Gly | M. L. Vasil, University of Colorado Denver (Barton et al., 1996) |
PAO6261 | PAO1 parent strain; Δanr | M. L. Vasil (Barton et al., 1996) |
PAO1 : : pvdS | PAO1 with 460 bp pvdS internal deletion, Gmr | I. Lamont, University of Otago, New Zealand (Cunliffe et al., 1995) |
PW6979 : : bfrB-lacZ | bfrB-C04 : : ISlacZ/hah, Tcr | University of Washington Genome Center (Jacobs et al., 2003) |
Plasmids | ||
pRL88 | pSW205 carrying a regA–lacZ fusion, Cbr | D. Storey, University of Calgary, Canada (Storey et al., 1990) |
pMP220 : : PpvdA | pMP220 carrying a pvdA promoter transcriptional lacZ fusion, Tcr | P. Visca, University ‘Roma Tre’, Italy (Ambrosi et al., 2002) |
pMP220 : : PpvdS | pMP220 carrying a pvdS promoter transcriptional lacZ fusion, Tcr | P. Visca (Ambrosi et al., 2002) |
pDH10 | pMP190 carrying a pchR–lacZ fusion, Smr Cmr | K. Poole, Queen’s University, Canada (Heinrichs & Poole, 1996) |
Cb, carbenicillin; Cm, chloramphenicol; Gm, gentamicin; Sm, streptomycin; Tc, tetracycline; r, resistant.