Table 2. PAO1 iron-controlled genes influenced by serum in the early exponential phase of growth.
| ORF* | Gene* | Function/class* | Fold change† | Fur box (FB)GATAATGATAATCATTATC‡Iron starvation box (ISB)TAAAT-N16-CGT§ |
| PA0707 | toxR/regA | Transcriptional regulator ToxR/RegA | 14 | |
| PA1317 | cyoA | Cytochrome o ubiquinol oxidase subunit II | 11 | |
| PA2384 | nn|| | Probable DNA-binding protein | 27 | |
| Pyoverdine synthesis and transport | ||||
| PA2385 | pvdQ | PvdQ | 18 | |
| PA2386 | pvdA | l-Ornithine N5-oxygenase | 90 | ISB |
| PA2389 | pvdR | Protein of unknown function | 27 | ISB |
| PA2390 | pvdT | ABC transporter | 20 | |
| PA2391 | opmQ | Probable outer-membrane protein | 20 | |
| PA2392 | pvdP | PvdP | 70 | ISB |
| PA2393 | nn | Probable dipeptidase precursor | 59 | ISB |
| PA2394 | pvdN | PvdN | 89 | |
| PA2395 | pvdO | PvdO | 24 | |
| PA2396 | pvdF | Pyoverdine synthetase F | 92 | ISB |
| PA2397 | pvdE | Pyoverdine biosynthesis protein PvdE | 26 | ISB |
| PA2398 | fpvA | Ferripyoverdine receptor | 28 | |
| PA2399 | pvdD | Pyoverdine synthetase D | 30 | |
| PA2413 | pvdH | Diaminobutyrate-2-oxoglutarate aminotransferase | 17 | ISB |
| PA2425 | pvdG | PvdG | 51 | |
| PA2426 | pvdS | Sigma factor PvdS | 37 | FB (GtaAtTGAcAATCATTATC) |
| PA3531 | bfrB | Bacterioferritin | 0.09 | Fur-like binding sequence |
| PA4159 | fepB | Ferrienterobactin-binding periplasmic protein precursor | 9 | |
| Pyochelin synthesis and transport | ||||
| PA4168 | fpvB | Second ferric pyoverdine receptor FpvB | 10 | |
| PA4218 | fptX | Putative permease, AmpG paralogue | 5.3 | |
| PA4219 | fptC | Hypothetical protein | 5.8 | |
| PA4220 | fptB | Hypothetical protein | 6.2 | |
| PA4221 | fptA | Fe(III)-pyochelin outer-membrane receptor precursor | 10 | FB (cATAATGATAAgCATTATC) |
| PA4222 | pchI | Probable ATP-binding component of ABC transporter | 5.65 | |
| PA4223 | pchH | Probable ATP-binding component of ABC transporter | 3.12 | |
| PA4225 | pchF | Pyochelin synthetase | 2.5 | |
| PA4226 | pchE | Dihydroaeruginoic acid synthetase | 8 | |
| PA4227 | pchR | Transcriptional regulator PchR | 13 | FB (GgaAATGAgAtTtATTATC) |
| PA4228 | pchD | Pyochelin biosynthesis protein D | 22 | |
| PA4229 | pchC | Pyochelin biosynthesis protein C | 18 | |
| PA4230 | pchB | Salicylate biosynthesis protein PchB | 18 | |
| PA4231 | pchA | Salicylate biosynthesis isochorismate synthase | 9 | |
| PA4359 | feoA | Ferrous iron transport protein A | 11.5 | |
| PA4468 | sodM | Superoxide dismutase | 13 | FB (GATAATGAgAtTgATTATt) |
| PA4469 | unc¶ | Hypothetical protein | 46 | |
| PA4470 | fumC1 | Fumarate hydratase | 68 | |
| PA4471 | unc | Hypothetical protein | 90 | |
| Haem transport and utilization | ||||
| PA4705 | phuW | Hypothetical protein | 4.6 | |
| PA4706 | phuV | Haemin importer ATP binding subunit | 7.9 | |
| PA4707 | phuU | Permease of ABC transporter | 12.5 | |
| PA4708 | phuT | Haem-transport protein | 21 | FB (GcTAATGcaAATaATTATC) |
| PA4709 | phuS | Haemin degrading factor | 20 | |
| PA4710 | phuR | Haem/haemoglobin uptake outer membrane receptor precursor | 32 | FB (GATAATtATttgCATTAgC) |
| PA5531 | tonB1 | TonB1 protein | 6 | FB (ctgAATGATAATaATTATC) |
*
Information obtained from the Pseudomonas Genome DatabaseV2 (http://www.pseudomonas.com/) (Winsor et al., 2009).
†
PAO1 grown in TSB-DC/ABS compared to PAO1 grown in TSB-DC.
‡
Consensus sequence for Fur box (Ochsner et al., 2002).
§
Consensus sequence for iron starvation box (Wilson et al., 2001).
||
Not named.
¶
Unclassified.