Table 1.

Human gene sequences for IL-1α and GAPDH, PCR annealing temperatures and number of amplification cycles

Gene analyzed	Human sequences	Annealing temp. (°C)	Cycle no.
Gel-based PCR
IL-1α	F: AAG CCT TCC TGC CGC AAC	57	32
	R: CTG CAC CTA CCA AAC ACG G
GAPDH	F: AGG TCG GAG TCA ACG GAT TTG	57	32
	R: TGG CAG GTT TTT CTA GAC GGC