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The Tyr127 residue of Tip is required for the interaction with and phosphorylation of STAT6. Jurkat T-cells were electroporated with GFP, GFP–Tip or GFP–Tip mutant expression constructs. Twenty-four hours after electroporation, cells were lysed and used for immunoprecipitation (IP) with an antibody against GFP, followed by immunoblotting with the indicated antibodies. Whole-cell lysates (WCL) were used to detect the expression level of STATs and Lck.
