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. 2012 Feb;93(Pt 2):347–355. doi: 10.1099/vir.0.035113-0

Fig. 1.

Fig. 1.

Native dot-blot analysis of AAV serotype cross-reactivity with the anti-AAV1 and anti-AAV5 mAbs. (a) At standard concentrations (hybridoma supernatant diluted 1 : 100), the anti-AAV1 antibodies (4E4, 5H7 and 9A8) cross-react only with AAV6 capsids and no cross-reactivity is seen with the anti-AAV5 antibodies (3C5, 8F1 and 9F7). The B1 and ADK4 antibodies are positive controls for the presence of VLPs. (b) Cross-reactivity of AAV5 antibodies for AAV1 capsids is observed only when tested at higher concentrations (supernatant diluted 1 : 4).