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. 2012 Feb;93(Pt 2):389–399. doi: 10.1099/vir.0.036566-0

Fig. 2.

Fig. 2.

Detection and size distribution of AcMNPV and Ac-FPm BV DNA linearized with AvrII at different passages by PFGE. DNA was obtained from purified virions. (a) Comparison of DIP DNA formation in AcMNPV and Ac-FPm at passages 1, 16, 19, 25 and 28. (b) Reduced DIP DNA production in Ac-FPm compared with WT AcMNPV at early passages (passage 5, 9 and 12). The arrow indicates the defective genome d37 with 37 % of the genome deleted. (c) Size distribution of the standard and defective genomes of WT AcMNPV and Ac-FPm at passage 32. Lanes: L, MidRange I PFG ladder; L1, λ DNA ladder, with sizes shown in kb in (a); S, whole Salmonella DNA digested with XbaI (used as a control for electrophoresis and size marker); W, WT AcMNPV DNA; FPm, Ac-FPm DNA. Arrows indicate sizes (kb) of DIP DNAs.