Table 1. Nucleotide sequence comparisons of LBPR-0379 HVR populations.
Source | No. nucleotides inserted (position)*/deleted (position) | No. clones | Clones (%) |
Faeces (~800 bp) | 0/0 | 22/37 | 59.5 |
117 (2189)/0 | 13/37 | 35.0 | |
0/770 (2031) | 2/37 | 5.4 | |
(~200 bp) | 0/770 (2031) | 54/55 | 98.2 |
0/801 (2018) | 1/55 | 1.8 | |
Passage 1, faecal (~800 bp) | 117 (2189)/0 | Consensus† | |
(~500 bp) | 117 (2189)/452 (2343) | Consensus | |
Passage 2, faecal (~800 bp) | 0/463 (2356) | 2/15 | 13.3 |
117 (2189)/0 | 10/15 | 66.7 | |
0/760 (1952) | 2/15 | 13.3 | |
0/828 (1991) | 1/15 | 6.7 | |
Serum (~800 bp) | 117(2189)/0 | 44/44 | 100 |
Passage 1, serum (~800 bp) | 117 (2189)/42 (2381) | 92/92 | 100 |
Numbering according to the sequence with the GenBank accession no. JN564006 which contains the 117 nt insert at position 2189.
PCR products were purified on agarose gels, sequenced directly and the sequence was deposited in GenBank as JQ036303–JQ036304. The consensus sequence for underlined clones was deposited in GenBank as JQ036300–JQ036302, JQ036305–JQ036307. Clones not underlined were shorter than the 200 nt minimum required for deposit in GenBank.