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Determination of CypA insertion. (a) Diagram indicating the splicing of TRIM5α or TRIMCyp. Non-coding and coding exons (numbered) and CypA sequences are indicated as open, shaded and filled boxes, respectively. The primers used in this study are indicated by arrows. (b) Genomic DNA was extracted from PBMCs. To test for CypA insertion, the 3′ region of the TRIM5 gene was amplified by PCR with primers spanning the 3′ UTR and the putative CypA insertion. DW, Distilled water control.
