Fig. 5.

Relative transcription ratios of the chitinase-encoding genes nag1 and ech42, and the protease-encoding gene prb1 in transformants gpr1 sil-7 (grey bars) and sil-8 (black bars) and the parental strain (white bars) during confrontation with R. solani. Samples were taken from a control in which the parental strain and the gpr1-silenced transformants were grown alone and directly after contact with R. solani as host and subjected to real-time RT-PCR using sar1 as reference gene. The control sample of the parental strain was arbitrarily assigned the value 1. Results are means±sd (n = 3).