Fig. 6.

Relative transcription ratios of the chitinase-encoding genes ech42 (a) and nag1 (b) in transformant gpr1 sil-8 (black bars) and the parental strain (white bars). Real-time RT-PCR was performed 5, 12 and 20 h after transfer of the mycelia to liquid growth medium containing 1 % N-acetylglucosamine (NAG), and 24, 36, 48 and 60 h after transfer to medium containing 1 % colloidal chitin (Chit), using act1 as reference gene. Samples of the parental strain at NAG 5 h and Chit 24 h were arbitrarily assigned the value 1. To all values 1 was added to allow illustration on a logarithmic scale. Results are means±sd (n = 3).