Fig. 7.

Confocal microscopic studies of the interaction of the T. atroviride parental strain (WT, a) and the gpr1-silenced transformant sil-7 (b) with R. solani. Co-cultures were incubated until contact between the two fungi and pictures (left, confocal images; right, bright-field images) were taken after staining with FM4-64. Arrows indicate hyphae of the T. atroviride parental strain and the gpr1-silenced transformant which are in contact with R. solani. Upon interaction with the parental strain (a), hyphae of R. solani are lysed, resulting in staining of the complete hyphal compartment of the dead hypha. Upon confrontation with the gpr1-silenced transformant (b), hyphae of R. solani remain completely intact, reflected by the stained intact hyphal membrane.