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. 2012 Jan;158(Pt 1):107–118. doi: 10.1099/mic.0.052035-0

Table 1. Oligonucleotides used in this study.

Gene/primer Forward primer (5′ to 3′) Reverse primer (5′ to 3′)
Primer pairs used for quantification of transcripts by real-time RT-PCR
gpr1 TTGATCCAGACCTTCATGCCAGC CATAAAAGGCCGCGACACGAA
nag1 TGTCCTACAGCCTCTGCTGCAAAAGTTC CATCTCCTCACAGACAAGCGGTGAAAG
ech42 CGCAACTTCCAGCCTCAGAACC TCAATACCATCGAAACCCCAGTCC
prb1 CGCACTGCTTCCTTCACCAACT TTTCACTTCATCCTTCGCTCCA
sar1 CTCGACAATGCCGGAAAGACCA TTGCCAAGGATGACAAAGGGG
act1 GCACGGAATCGCTCGTTG TTCTCCACCCCGCCAAGC
128908 TTCGTCGTTATTGCGTCCAC GGCAGCCTTCTTTCCATACC
90851 TCGCCGATTATCAGAAGG CGCATACAGAGCTGGATTG
146614 TTCTCGGCTCTTGGGAC CTCTTGCTGCTTGACTTGTG
132043 AAGACGACGACATCCGAGAC TGGAATCATTGCCGAACC
142851 TGGTGGTGACCGCGTTACAG TGGCGTTGAGCACTCCGTTG
137004 ATAATGCCTCCGCCTTCC CCGACTTGCCCAAATAGC
142792 AAAGGGGCCATGTCTATCAA GAAAAGGCAACTTCCTCAAA
160894 AAAGGAAGCGGAAAGAAGC GGAAATGAACATCACCGACC
145570 ATTCGCCGAGGATGAGAG CGTTGGGGTTTGACTGAG
133072 CACCGCTGCTGACAACAAGG GCCAGAAGGGCAGCAATGAC
159687 TCGCGTCCCATGATACC GGAAGTCGTTGAGCACCAG
138324 AACAGCGGATGGGACACG GGGAGATACCAAAGGAGGGAC
133633 CGAGCAAGTCATCAAGGTG TGGATAGGCTGCCAAAGTAG
142538 TGCGAAGACGATCCTAGAC CACAGACCAATCCAAACG
129518 GACACTTCCAGTCCAGCATC GGGAGGCGATTTGGTTAC
148150 CATAGCAGATGGCTCGTG GCGACAAAGTTCTGGTGC
Oligonucleotides used for gene amplification
tga1sfi TGGCCATTACGGCCATGGGTTGCGGAATGTCTACAG AGGCCGAGGCGGCCGCTAAATGAGACCGCATAAACG
tga2sfi TGGCCATTACGGCGATGTGCTTCGGGGCTC AGGCCGAGGCGGCCGTTATAGTATTAACTTTTTGAGG
tga3sfi TGGCCATTACGGCCATGGGCGGCTGCATGAGC AGGCCGAGGCGGCCGTTAAAGAATACCCGAATC
tga1dual TGGCCATTACGGCCAGATGGGTTGCGGAATGTCTAC GAGGCCGAGGCGGCCATGAGACCGCATAAACGAAGG
tga2dual TGGCCATTACGGCCCAATGTGCTTCGGGGCTC GAGGCCGAGGCGGCCAGTATTAACTTTTTGAG
tga3dual TGGCCATTACGGCCCCATGGGCGGCTGCAT GAGGCCGAGGCGGCCAGAATACCCGAATCC
tgpcr1sfi TGGCCATTACGGCCATGGCCGGAGGACTCTCAGAGG TGGCCGAGGCGGCCACTGCCTGCTCTGGAATTTCTGC
SdMGpr1 GATACCGTCGAGCGCCAAC CAGGTGATAGGCATGGCCGT