Primer pairs used for quantification of transcripts by real-time RT-PCR |
gpr1 |
TTGATCCAGACCTTCATGCCAGC |
CATAAAAGGCCGCGACACGAA |
nag1 |
TGTCCTACAGCCTCTGCTGCAAAAGTTC |
CATCTCCTCACAGACAAGCGGTGAAAG |
ech42 |
CGCAACTTCCAGCCTCAGAACC |
TCAATACCATCGAAACCCCAGTCC |
prb1 |
CGCACTGCTTCCTTCACCAACT |
TTTCACTTCATCCTTCGCTCCA |
sar1 |
CTCGACAATGCCGGAAAGACCA |
TTGCCAAGGATGACAAAGGGG |
act1 |
GCACGGAATCGCTCGTTG |
TTCTCCACCCCGCCAAGC |
128908 |
TTCGTCGTTATTGCGTCCAC |
GGCAGCCTTCTTTCCATACC |
90851 |
TCGCCGATTATCAGAAGG |
CGCATACAGAGCTGGATTG |
146614 |
TTCTCGGCTCTTGGGAC |
CTCTTGCTGCTTGACTTGTG |
132043 |
AAGACGACGACATCCGAGAC |
TGGAATCATTGCCGAACC |
142851 |
TGGTGGTGACCGCGTTACAG |
TGGCGTTGAGCACTCCGTTG |
137004 |
ATAATGCCTCCGCCTTCC |
CCGACTTGCCCAAATAGC |
142792 |
AAAGGGGCCATGTCTATCAA |
GAAAAGGCAACTTCCTCAAA |
160894 |
AAAGGAAGCGGAAAGAAGC |
GGAAATGAACATCACCGACC |
145570 |
ATTCGCCGAGGATGAGAG |
CGTTGGGGTTTGACTGAG |
133072 |
CACCGCTGCTGACAACAAGG |
GCCAGAAGGGCAGCAATGAC |
159687 |
TCGCGTCCCATGATACC |
GGAAGTCGTTGAGCACCAG |
138324 |
AACAGCGGATGGGACACG |
GGGAGATACCAAAGGAGGGAC |
133633 |
CGAGCAAGTCATCAAGGTG |
TGGATAGGCTGCCAAAGTAG |
142538 |
TGCGAAGACGATCCTAGAC |
CACAGACCAATCCAAACG |
129518 |
GACACTTCCAGTCCAGCATC |
GGGAGGCGATTTGGTTAC |
148150 |
CATAGCAGATGGCTCGTG |
GCGACAAAGTTCTGGTGC |
Oligonucleotides used for gene amplification |
tga1sfi |
TGGCCATTACGGCCATGGGTTGCGGAATGTCTACAG |
AGGCCGAGGCGGCCGCTAAATGAGACCGCATAAACG |
tga2sfi |
TGGCCATTACGGCGATGTGCTTCGGGGCTC |
AGGCCGAGGCGGCCGTTATAGTATTAACTTTTTGAGG |
tga3sfi |
TGGCCATTACGGCCATGGGCGGCTGCATGAGC |
AGGCCGAGGCGGCCGTTAAAGAATACCCGAATC |
tga1dual |
TGGCCATTACGGCCAGATGGGTTGCGGAATGTCTAC |
GAGGCCGAGGCGGCCATGAGACCGCATAAACGAAGG |
tga2dual |
TGGCCATTACGGCCCAATGTGCTTCGGGGCTC |
GAGGCCGAGGCGGCCAGTATTAACTTTTTGAG |
tga3dual |
TGGCCATTACGGCCCCATGGGCGGCTGCAT |
GAGGCCGAGGCGGCCAGAATACCCGAATCC |
tgpcr1sfi |
TGGCCATTACGGCCATGGCCGGAGGACTCTCAGAGG |
TGGCCGAGGCGGCCACTGCCTGCTCTGGAATTTCTGC |
SdMGpr1 |
GATACCGTCGAGCGCCAAC |
CAGGTGATAGGCATGGCCGT |