Table 3. Summary of overall mean titres in DEF cells at 37, 41 and 44 °C.

Horizontal lines indicate the three groups of data from independent experiments.

Virus strain	Overall mean virus titre (p.f.u. ml−1)
	37 °C*	41 °C*	44 °C*	Δ37–41†	Δ41–44†	Δ37–44†
COAV997	7.5a	6.9a	1.7a	0.6	5.2	5.8
COAV997-IC	7.5a	6.9a	2.2a	0.6	4.7	5.3
IMP102	7.8b	7.3a	5.1b	0.5	2.2	2.7
CA-04	7.8b	7.2a	6.0b	0.6	1.2	1.8
COAV2900	7.9b	7.2a	5.6b	0.7	1.6	2.3
NY99	7.8b	7.1a	5.5b	0.7	1.6	2.3
COAV997	7.7ab	7.5a	1.9a	0.2	5.6	5.8
COAV997-IC	7.5a	7.3a	2.9b	0.2	4.4	4.6
E	7.6a	7.5a	6.0c	0.1	1.5	1.6
NS1	7.4a	7.4a	5.5c	0	1.9	1.9
E.NS1	7.5a	7.5a	5.7c	0	1.8	1.8
3′ UTR	8.0b	7.6a	5.9c	0.4	1.7	2.1
NY99	7.9b	7.4a	6.0c	0.5	1.4	1.9
COAV997	8.1a	7.4b	1.7a	0.7	5.7	6.4
COAV997-IC	8.0a	7.2ab	2.6a	0.8	4.6	5.4
NS1	7.8a	7.1a	5.9c	0.7	1.2	1.9
NS4A	8.1a	7.0a	5.4bc	1.1	1.6	2.7
NS1.NS4A	7.9a	7.0a	1.9a	0.9	5.1	6.0
E.NS1.3′UTR	8.0a	7.4b	4.3b	0.6	3.1	3.7
NY99	8.1a	7.2ab	6.3c	0.9	0.9	1.8

^*Means followed by the same superscripts (a, b and c) were not significantly different by pairwise analysis (Tukey–Kramer multiple comparisons test, α<0.05).

^†The difference in overall mean titre between 37–41 °C, 41–44 °C and 37–44 °C, as indicated.