. 2009 Feb 2;2(2):222–233. doi: 10.1111/j.1752-4571.2008.00065.x

Table 2.

Hierarchical AMOVA calculated in GenAlEx with respect to different alleles (F_ST) and the sum of squared size differences of the alleles (R_ST)

	Scott	Rowleys	Dampier	Ningaloo	All
F_SR: within systems	0.020^***	0.025^***	0.007^*	0.016^***	0.017^***
F_RT^A: among systems					0.046^***
F_RT^B: among zones					0.041^***
F_ST: among all sites					0.062^***
R_SR: within systems	0.021^***	0.031^***	0.019^**	0.011^**	0.020^***
R_RT^A: among systems					0.072^***
R_RT^B: among zones					0.070^***
R_ST : among all sites					0.090^***

The proportion of variance was estimated in two steps: first, among sites within systems (F_SR and R_SR) calculated with data from each system only and with data from all northwest Australian sites; and second, among systems (F_RT^A and R_RT^A), between coastal (Dampier and Ningaloo) and offshore (Scott and Rowleys) zones (F_RT^B and R_RT^B) and among all sites (F_ST and R_ST) relative to the total variance of all the northwest Australian sites.

Tests for statistical significance were based on 1000 random permutations. Levels of statistical significance for the F- and R- values are indicated by ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.