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. 2008 Oct 10;1(4):631–644. doi: 10.1111/j.1752-4571.2008.00048.x

Table 3.

NCA results for all significant clades

Nested clade permutation analysis

Clade 3-1 (observed χ2-statistic = 466.0855, P < 0.0001)

Type of distance Distance PSS PSL
Clade 2-1 (Interior)
 Within clade 753.3448 0.0000 1.0000
 Nested clade 1099.3743 0.1480 0.8520
Clade 2-2 (Tip)
 Within clade 1135.2346 0.3770 0.6230
 Nested clade 1186.7130 0.7290 0.2710
Interior versus tip clades I-T distance PSS PSL




 Within clade −381.8898 0.0010 0.9990
 Nested clade −87.3387 0.2030 0.7970
Inference = range expansion for clades 2-1 and 2-2
Clade 2-1 (observed χ2-statistic = 91.6938, P < 0.0001)

Type of distance Distance PSS PSL




Clade 1-1 (interior)
 Within clade 692.0376 0.1230 0.8770
 Nested clade 865.0450 1.0000 0.0000
Clade 1-2 (Tip)
 Within clade 84.2306 0.0000 1.0000
 Nested clade 613.7558 0.0000 1.0000
Clade 1-3 (Tip)
 Within clade 0.0000 1.0000 1.0000
 Nested clade 691.1035 0.9220 0.3240
Interior versus tip clades I-T distance PSS PSL




 Within clade 608.5208 1.0000 0.0000
 Nested clade 250.6338 1.0000 0.0000
Inference = isolation by distance
Clade 2-3 (Observed χ2-statistic = 102.4387, P < 0.0001)

Type of distance Distance PSS PSL




Clade 1-5 (interior)
 Within clade 2071.4351 0.9480 0.0520
 Nested clade 2062.5834 0.9060 0.0940
Clade 1-6 (Tip)
 Within clade 1546.9352 0.0440 0.9560
 Nested clade 1711.8136 0.0760 0.9240
Interior versus tip clades I-T distance PSS PSL




 Within clade 524.5000 0.9580 0.0420
 Nested clade 350.7698 0.9230 0.0770
Inference = isolation by distance
Clade 1-5 (observed χ2-statistic = 762.4985, P < 0.0001)

Type of distance Distance PSS PSL




Clade IA (Interior)
 Within clade 1903.6805 0.0000 1.0000
 Nested clade 1974.1171 0.0000 1.0000
Clade In1 (Tip)
 Within clade 0.0000 0.0730 1.0000
 Nested clade 602.0199 0.0320 0.9880
Clade IE (tip)
 Within clade 0.0000 1.0000 1.0000
 Nested clade 1875.2362 0.4620 0.5970
Clade IF (Tip)
 Within clade 0.0000 1.0000 1.0000
 Nested clade 2015.3519 0.6390 0.4420
Clade In3 (tip)
 Within clade 0.0000 1.0000 1.0000
 Nested clade 602.0199 0.1680 0.9670
Clade In2 (Tip)
 Within clade 0.0000 0.0010 1.0000
 Nested clade 1923.7367 0.4870 0.5130
Clade IG (tip)
 Within clade 0.0000 1.0000 1.0000
 Nested clade 3020.2976 0.8230 0.1940
Clade IB (Tip)
 Within clade 0.0000 0.0000 1.0000
 Nested clade 1711.7679 0.2560 0.7440
Clade IC (tip)
 Within clade 0.0000 0.0010 1.0000
 Nested clade 3629.0913 1.0000 0.0000
Clade IH (Tip)
 Within clade 0.0000 1.0000 1.0000
 Nested clade 2975.8615 0.8290 0.2050
Interior versus tip clades I-T distance PSS PSL




 Within clade 1903.6805 1.0000 0.0000
 Nested clade −160.3560 0.1260 0.8740
Inference = restricted gene flow/dispersal with some long distance dispersal for clade IC.

Within and nested clade distances are given, in miles, for both interior (I) and tip (T) clades. PSS, P-value for a significantly small value; PSL, P-value for a significantly large value. I-T distance is the interior clade distance minus the tip clade distance. Inferences based on Templeton key are given for each clade.